Gel Extraction kit offers a quick, convenient, and economical way to isolate DNA fragments from standard or low-melt Agarose gels following gel analysis. As a result of the buffer system included in the kit, DNA fragments that have been solubilized on gel slices easily bind to the spin column. The DNA samples are washed using wash buffers in order to remove agarose gel, salts, and other impurities. The purified DNA is eluted in small volumes for further processing. Each purification column has a total binding capacity of up to 20 μg of DNA and the entire procedure takes less than 15 min.

A DNA fragment is excised from an agarose gel, placed in a microcentrifuge tube, dissolved in binding buffer, and kept in a dry bath. One volume of Isopropanol will be added upon complete dissolution of agarose gel. In the Gel Extraction Buffer, chaotropic agents are added, which dissolve agarose, denature proteins, and help DNA bind to the silica membrane. A simple wash step removes impurities. Using the elution buffer, purified DNA is eluted from the column. Following the recovery of DNA, downstream applications can be performed.