DNA clean-up kit is a fast, convenient and economical way to purify DNA fragments (50 bp – 10 Kb). In less than ten minutes, our product allows you to complete a rapid protocol. A PCR reaction mixture, enzyme mixture, or other reaction mixture that contains primers, dNTPs, and unincorporated labeled nucleotides is removed by this method. Furthermore, it uses a silica-based membrane technology, which facilitates easy resin manipulations and eliminates the need for toxic phenol-chloroform extractions.

In a purification column, the DNA is mixed with a binding buffer. A chaotropic agent in the binding buffer denatures proteins and promotes DNA binding to the silica membrane in the column. Impurities can be removed with a simple wash step. Following the purification of the DNA, the elution buffer is used to elute the purified DNA. Using the recovered DNA, downstream applications can be performed.